Cas12a Mutant Structural Predictions With AlphaFold2

Materials designed for class CHBE0165 (Biomolecular Engineering and Design) Spring 2024.

Content developed by TTS Research Technology

• Shirley Li, PhD, Bioinformatician, xue.li37@tufts.edu
• Jason Laird, MSc, former Bioinformatics Scientist

The Research Technology Team

• Consultation on Projects and Grants
• High Performance Compute Cluster
• Workshops

Prerequisites

• Download PyMOL
• Account on the HPC (Note: To test your account log into OnDemand. If you can log in, you are all set, if not please reach out to tts-research@tufts.edu)

Contents

1. Run AlphaFold2 on Tufts HPC CLI
2. Run AlphaFold2 on Tufts HPC with Open OnDemand and Accuracy Assessment
3. Vizualize Predicted Structure with PYMOL
4. Slides for this lecture is here.

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