Next Generation Sequencing (NGS) Data Analysis on the Tufts HPC
Description
This is Day 2 of the High Performance Computing For Life Sciences workshop. Here we examine how to download publically available NGS data, perform quality control on that data, produce and alignment and view reads and variants.
Goals
Materials Needed
- Chrome web browser
- Account on Tufts High Performance Compute Cluster
- VPN if accessing the HPC from off campus
Prerequisite
Table of Contents
- Background (10 min)
- Logging In (5 min)
- Obtain Data (15 min)
- Quality Control (15 min)
- Process Reads (15 min)
- Aligning NGS reads (30 min)
- Viewing NGS read alignments (15 min)
- Conclusion