Introduction Amplicon Sequencing Analysis

Description

This tutorial introduces microbial amplicon sequence analysis. We will explore what amplicon sequencing is, how to perform quality control, how to obtain abundance tables, and finally how to analyze our results.

Materials Needed

• Chrome web browser
• Account on Tufts HPC
• VPN if accessing the HPC from off campus

Prerequisites

• Intro To HPC
• Intro To R

Schedule

• Introduction to Amplicon Sequencing
• Setup
• Quality Control
• Sequence Error Mdoel/Dereplication
• Inferring ASVs/Merging Reads
• Creating the Count Table/Chimera Removal
• Assign Taxonomy
• Data Analysis

Acknowledgement

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